Genetic and phenotypic variation of Phytophthora crassamura isolates from California nurseries and restoration sites.
Identifieur interne : 000499 ( Main/Exploration ); précédent : 000498; suivant : 000500Genetic and phenotypic variation of Phytophthora crassamura isolates from California nurseries and restoration sites.
Auteurs : Laura L. Sims [États-Unis] ; Cameron Chee [États-Unis] ; Tyler Bourret [États-Unis] ; Shannon Hunter [Nouvelle-Zélande] ; Matteo Garbelotto [États-Unis]Source :
- Fungal biology [ 1878-6146 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- génétique : ADN fongique, Phytophthora.
- Californie, Génotype, Phylogenèse, Phénotype, Variation génétique, Variation intra-population.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Fungal.
- geographic : California.
- genetics : Phytophthora.
- Biological Variation, Population, Genetic Variation, Genotype, Phenotype, Phylogeny.
Abstract
Phenotypic and sequence data were used to characterize 28 isolates resembling Phytophthora megasperma from 14 host species in 2 plant production facilities and 10 restoration sites across the San Francisco Bay Area (California; USA). Size of the oogonia and DNA sequences (nuclear internal transcribed spacer (ITS) and mitochondrial cytochrome c oxidase subunit 1 (COX 1)) were compared, and sensitivity to mefenoxam and pathogenicity were measured. Based on ITS 61 % of isolates matched ex-type sequences of Phytophthora crassamura from Italy, and the remainder matched or were close to the P. megasperma ex-type. However, all California P. crassamura genotypes belonged to four unique COX 1 haplotype lineages isolated from both nurseries and restoration sites. Although lineages were sensitive to mefenoxam, a significant difference in sensitivity was identified, and all continued growth in-vitro. These results suggested previous mefenoxam exposure in plant production facilities resulting in tolerance. In conclusion, all evidence pointed to a nursery origin of novel P. crassamura lineages found in California restoration sites. In this study, COX 1 sequences and oogonia size provided information relevant to identify geographic and evolutionary intraspecific variation within P. crassamura, and was additionally used to track the spread of this species from nurseries into wildlands.
DOI: 10.1016/j.funbio.2018.11.012
PubMed: 30709521
Affiliations:
Links toward previous steps (curation, corpus...)
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Sims</name><affiliation wicri:level="1"><nlm:affiliation>Department of Environmental Science, Policy and Management, University of California, Berkeley, CA, 94720, USA; Forestry Program, School of Agricultural Sciences and Forestry, Louisiana Tech University, LA, 71272, USA. Electronic address: simsla@latech.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Environmental Science, Policy and Management, University of California, Berkeley, CA, 94720, USA; Forestry Program, School of Agricultural Sciences and Forestry, Louisiana Tech University, LA, 71272</wicri:regionArea><wicri:noRegion>71272</wicri:noRegion></affiliation></author><author><name sortKey="Chee, Cameron" sort="Chee, Cameron" uniqKey="Chee C" first="Cameron" last="Chee">Cameron Chee</name><affiliation wicri:level="1"><nlm:affiliation>Department of Environmental Science, Policy and Management, University of California, Berkeley, CA, 94720, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Environmental Science, Policy and Management, University of California, Berkeley, CA, 94720</wicri:regionArea><wicri:noRegion>94720</wicri:noRegion></affiliation></author><author><name sortKey="Bourret, Tyler" sort="Bourret, Tyler" uniqKey="Bourret T" first="Tyler" last="Bourret">Tyler Bourret</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, University of California, Davis, CA, 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology, University of California, Davis, CA, 95616</wicri:regionArea><wicri:noRegion>95616</wicri:noRegion></affiliation></author><author><name sortKey="Hunter, Shannon" sort="Hunter, Shannon" uniqKey="Hunter S" first="Shannon" last="Hunter">Shannon Hunter</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, School of Science, University of Waikato, Forest Protection, Scion, Rotorua 3010, New Zealand.</nlm:affiliation><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>Department of Biology, School of Science, University of Waikato, Forest Protection, Scion, Rotorua 3010</wicri:regionArea><wicri:noRegion>Rotorua 3010</wicri:noRegion></affiliation></author><author><name sortKey="Garbelotto, Matteo" sort="Garbelotto, Matteo" uniqKey="Garbelotto M" first="Matteo" last="Garbelotto">Matteo Garbelotto</name><affiliation wicri:level="1"><nlm:affiliation>Department of Environmental Science, Policy and Management, University of California, Berkeley, CA, 94720, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Environmental Science, Policy and Management, University of California, Berkeley, CA, 94720</wicri:regionArea><wicri:noRegion>94720</wicri:noRegion></affiliation></author></analytic><series><title level="j">Fungal biology</title><idno type="ISSN">1878-6146</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biological Variation, Population (MeSH)</term><term>California (MeSH)</term><term>DNA, Fungal (genetics)</term><term>Genetic Variation (MeSH)</term><term>Genotype (MeSH)</term><term>Phenotype (MeSH)</term><term>Phylogeny (MeSH)</term><term>Phytophthora (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (génétique)</term><term>Californie (MeSH)</term><term>Génotype (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Phytophthora (génétique)</term><term>Phénotype (MeSH)</term><term>Variation génétique (MeSH)</term><term>Variation intra-population (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Fungal</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>California</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN fongique</term><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biological Variation, Population</term><term>Genetic Variation</term><term>Genotype</term><term>Phenotype</term><term>Phylogeny</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Californie</term><term>Génotype</term><term>Phylogenèse</term><term>Phénotype</term><term>Variation génétique</term><term>Variation intra-population</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phenotypic and sequence data were used to characterize 28 isolates resembling Phytophthora megasperma from 14 host species in 2 plant production facilities and 10 restoration sites across the San Francisco Bay Area (California; USA). Size of the oogonia and DNA sequences (nuclear internal transcribed spacer (ITS) and mitochondrial cytochrome c oxidase subunit 1 (COX 1)) were compared, and sensitivity to mefenoxam and pathogenicity were measured. Based on ITS 61 % of isolates matched ex-type sequences of Phytophthora crassamura from Italy, and the remainder matched or were close to the P. megasperma ex-type. However, all California P. crassamura genotypes belonged to four unique COX 1 haplotype lineages isolated from both nurseries and restoration sites. Although lineages were sensitive to mefenoxam, a significant difference in sensitivity was identified, and all continued growth in-vitro. These results suggested previous mefenoxam exposure in plant production facilities resulting in tolerance. In conclusion, all evidence pointed to a nursery origin of novel P. crassamura lineages found in California restoration sites. In this study, COX 1 sequences and oogonia size provided information relevant to identify geographic and evolutionary intraspecific variation within P. crassamura, and was additionally used to track the spread of this species from nurseries into wildlands.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30709521</PMID><DateCompleted><Year>2019</Year><Month>07</Month><Day>04</Day></DateCompleted><DateRevised><Year>2019</Year><Month>07</Month><Day>04</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1878-6146</ISSN><JournalIssue CitedMedium="Internet"><Volume>123</Volume><Issue>2</Issue><PubDate><Year>2019</Year><Month>02</Month></PubDate></JournalIssue><Title>Fungal biology</Title><ISOAbbreviation>Fungal Biol</ISOAbbreviation></Journal><ArticleTitle>Genetic and phenotypic variation of Phytophthora crassamura isolates from California nurseries and restoration sites.</ArticleTitle><Pagination><MedlinePgn>159-169</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S1878-6146(18)30205-8</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.funbio.2018.11.012</ELocationID><Abstract><AbstractText>Phenotypic and sequence data were used to characterize 28 isolates resembling Phytophthora megasperma from 14 host species in 2 plant production facilities and 10 restoration sites across the San Francisco Bay Area (California; USA). Size of the oogonia and DNA sequences (nuclear internal transcribed spacer (ITS) and mitochondrial cytochrome c oxidase subunit 1 (COX 1)) were compared, and sensitivity to mefenoxam and pathogenicity were measured. Based on ITS 61 % of isolates matched ex-type sequences of Phytophthora crassamura from Italy, and the remainder matched or were close to the P. megasperma ex-type. However, all California P. crassamura genotypes belonged to four unique COX 1 haplotype lineages isolated from both nurseries and restoration sites. Although lineages were sensitive to mefenoxam, a significant difference in sensitivity was identified, and all continued growth in-vitro. These results suggested previous mefenoxam exposure in plant production facilities resulting in tolerance. In conclusion, all evidence pointed to a nursery origin of novel P. crassamura lineages found in California restoration sites. In this study, COX 1 sequences and oogonia size provided information relevant to identify geographic and evolutionary intraspecific variation within P. crassamura, and was additionally used to track the spread of this species from nurseries into wildlands.</AbstractText><CopyrightInformation>Copyright © 2018 British Mycological Society. Published by Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sims</LastName><ForeName>Laura L</ForeName><Initials>LL</Initials><AffiliationInfo><Affiliation>Department of Environmental Science, Policy and Management, University of California, Berkeley, CA, 94720, USA; Forestry Program, School of Agricultural Sciences and Forestry, Louisiana Tech University, LA, 71272, USA. Electronic address: simsla@latech.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chee</LastName><ForeName>Cameron</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Environmental Science, Policy and Management, University of California, Berkeley, CA, 94720, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bourret</LastName><ForeName>Tyler</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, University of California, Davis, CA, 95616, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hunter</LastName><ForeName>Shannon</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Biology, School of Science, University of Waikato, Forest Protection, Scion, Rotorua 3010, New Zealand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Garbelotto</LastName><ForeName>Matteo</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Environmental Science, Policy and Management, University of California, Berkeley, CA, 94720, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>12</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Fungal Biol</MedlineTA><NlmUniqueID>101524465</NlmUniqueID></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004271">DNA, Fungal</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000073537" MajorTopicYN="Y">Biological Variation, Population</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002140" MajorTopicYN="N" Type="Geographic">California</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="Y">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005838" MajorTopicYN="Y">Genotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="Y">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">COX 1 haplotypes</Keyword><Keyword MajorTopicYN="Y">Phytophthora megasperma</Keyword><Keyword MajorTopicYN="Y">Plant nurseries</Keyword><Keyword MajorTopicYN="Y">Wildland pathogens</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>08</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>10</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>11</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>2</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>2</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>7</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30709521</ArticleId><ArticleId IdType="pii">S1878-6146(18)30205-8</ArticleId><ArticleId IdType="doi">10.1016/j.funbio.2018.11.012</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Nouvelle-Zélande</li><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Sims, Laura L" sort="Sims, Laura L" uniqKey="Sims L" first="Laura L" last="Sims">Laura L. Sims</name></noRegion><name sortKey="Bourret, Tyler" sort="Bourret, Tyler" uniqKey="Bourret T" first="Tyler" last="Bourret">Tyler Bourret</name><name sortKey="Chee, Cameron" sort="Chee, Cameron" uniqKey="Chee C" first="Cameron" last="Chee">Cameron Chee</name><name sortKey="Garbelotto, Matteo" sort="Garbelotto, Matteo" uniqKey="Garbelotto M" first="Matteo" last="Garbelotto">Matteo Garbelotto</name></country><country name="Nouvelle-Zélande"><noRegion><name sortKey="Hunter, Shannon" sort="Hunter, Shannon" uniqKey="Hunter S" first="Shannon" last="Hunter">Shannon Hunter</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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